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Relative abundance and diversity of bacterial methanotrophs at the oxic-anoxic interface of the Congo deep-sea fan ArchiMer
Bessette, Sandrine; Moalic, Yann; Gautey, Sebastien; Lesongeur, Francoise; Godfroy, Anne; Toffin, Laurent.
Sitting at ~5 000 m water depth on the Congo-Angola margin and ~ 760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3-5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this study, we investigated relative abundance, diversity and distribution of aerobic methane-oxidizing bacteria (MOB) communities at the oxic-anoxic interface of sedimentary habitats by using fluorescence in situ hybridization and comparative sequence analysis of particulate mono-oxygenase (pmoA) genes. Our findings revealed that sedimentary habitats of the recent lobe complex hosted type I and type II MOB cells and...
Tipo: Text Palavras-chave: Aerobic methane-oxidizing bacteria; PmoA; Organic-rich sediment; Methane seeps; Congo deep-sea fan.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00382/49327/49706.pdf
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Subseafloor archaeal communities: from the surface to a deep hot biosphere? ArchiMer
Roussel, Erwan.
The sub-seafloor biosphere may contain two thirds of Earth's total prokaryotic biomass. The large and active microbial populations buried in the sub-seafloor play a key role in global biogeochemical cycles. However, little is known about these prokaryotic communities. The depth limit of this sub-seafloor biosphere is still unreached, and elevated temperatures as well as insufficient energy sources are the likely factors limiting life at depth. Interestingly, archaeal communities with unknown physiologies and no cultured relatives seem to have a key role in deep marine sediments and hydrothermal ecosystems. As most deep biosphere microorganisms detected so far have been extremely resistant to cultivation, molecular approaches remain to date the most...
Tipo: Text Palavras-chave: Sediment; Hydrothermal system; Ultramafic; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Deep biosphere; Sédiment; Écosystème hydrothermal; Ultramafique; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Biosphère profonde.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-4630.pdf
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